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CRY-tTA (B702)
(Plasmid #92032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherryN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4994
  • Total vector size (bp) 6830
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1836
  • Mutation
    NLS on CRY2 is mutated
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter CMV
  • Tag / Fusion Protein
    • tTA2 (TetR fused to VP64) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer custom
  • 3′ sequencing primer custom
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Modified from plasmid in Tucker lab (already available on Addgene, CRY2(deltaNLS)-mCh) which contains mCherry fluorescent protein in vector mCherryN1 (Clontech). tTA2 domain was amplified from Addgene 36430.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY-tTA (B702) was a gift from Chandra Tucker (Addgene plasmid # 92032 ; http://n2t.net/addgene:92032 ; RRID:Addgene_92032)

  • For your References section:

    Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Pathak GP, Spiltoir JI, Hoglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL. Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260. 10.1093/nar/gkx260 PubMed 28431041
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