CRY-GalVP16l (B694)
(Plasmid #92030)

• Purpose: expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)
• Depositing Lab: Chandra Tucker
• Publication: Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.1093/nar/gkx260.

Backbone

• Vector backbone: pmCherryN1
• Backbone size w/o insert (bp): 3950
• Total vector size (bp): 6487
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2
• Species: A. thaliana (mustard weed)
• Mutation: NLS sequence of CRY2 mutated
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV
• Tag / Fusion Protein:
  • GalBD-VP16 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry