p5lysAsfGFP
(Plasmid
#92025)
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PurposesfGFP reporter plasmid with lysA TALE binding site in its promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
- Total vector size (bp) 3712
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Vector typeTALEN
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP
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Insert Size (bp)717
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5lysAsfGFP was a gift from Brian Pfleger (Addgene plasmid # 92025 ; http://n2t.net/addgene:92025 ; RRID:Addgene_92025) -
For your References section:
A transcription activator-like effector (TALE) induction system mediated by proteolysis. Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF. Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8. 10.1038/nchembio.2021 PubMed 26854666