p5A1-T
(Plasmid
#92017)
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PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBT-2
- Total vector size (bp) 6765
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Modifications to backboneEncodes tetR.
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Vector typeTALEN
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameTALE targeting lac operator with 3 TEV cleavage sites
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Insert Size (bp)2598
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Tags
/ Fusion Proteins
- FLAG tag (C terminal on insert)
- 6x His Tag (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameTobacco Etch Virus Protease
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Alt nameTEV protease
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Insert Size (bp)750
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MutationS219V
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Tags
/ Fusion Proteins
- 7x His Tag (N terminal on insert)
- 5x Arginine Tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TEV protease induction will result in a growth defect if the culture is not outgrown to a suitable density prior to addition of anhydrotetracycline.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5A1-T was a gift from Brian Pfleger (Addgene plasmid # 92017 ; http://n2t.net/addgene:92017 ; RRID:Addgene_92017) -
For your References section:
A transcription activator-like effector (TALE) induction system mediated by proteolysis. Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF. Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8. 10.1038/nchembio.2021 PubMed 26854666