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Addgene

p5A1-T
(Plasmid #92017)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92017 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBT-2
  • Total vector size (bp) 6765
  • Modifications to backbone
    Encodes tetR.
  • Vector type
    TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    TALE targeting lac operator with 3 TEV cleavage sites
  • Insert Size (bp)
    2598
  • Tags / Fusion Proteins
    • FLAG tag (C terminal on insert)
    • 6x His Tag (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Tobacco Etch Virus Protease
  • Alt name
    TEV protease
  • Insert Size (bp)
    750
  • Mutation
    S219V
  • Tags / Fusion Proteins
    • 7x His Tag (N terminal on insert)
    • 5x Arginine Tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TEV protease induction will result in a growth defect if the culture is not outgrown to a suitable density prior to addition of anhydrotetracycline.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5A1-T was a gift from Brian Pfleger (Addgene plasmid # 92017 ; http://n2t.net/addgene:92017 ; RRID:Addgene_92017)
  • For your References section:

    A transcription activator-like effector (TALE) induction system mediated by proteolysis. Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF. Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8. 10.1038/nchembio.2021 PubMed 26854666