pCDB179
(Plasmid
#91960)
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Purpose(Empty Backbone) empty expression vector for 10His-SUMO tag protein purification
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 91960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDB179
- Backbone size (bp) 5552
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Modifications to backbonestarted with pET29 and exchanged the new multiple cloning site
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Vector typeBacterial Expression
- Promoter T7
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Tags
/ Fusion Proteins
- 10-His (N terminal on backbone)
- Smt3 (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCTGTATGACGGTATTCG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDB179 was a gift from Christopher Bahl (Addgene plasmid # 91960 ; http://n2t.net/addgene:91960 ; RRID:Addgene_91960)
Map uploaded by the depositor.
