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Addgene

MTS-mCherry-GFP1-10-Hyg-N1
(Plasmid #91957)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91957 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDsRed-Monomer-Hyg-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTS-mCherry-GFP1-10
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The MTS sequence is based on subunit 9 of F0-ATP synthase (pOLI1-HcRed) from Curr Biol. 2004 Nov 23;14(22):1996-2004

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MTS-mCherry-GFP1-10-Hyg-N1 was a gift from Rong Li (Addgene plasmid # 91957 ; http://n2t.net/addgene:91957 ; RRID:Addgene_91957)

  • For your References section:

    Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R. Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695. Epub 2017 Mar 1. 10.1038/nature21695 PubMed 28241148
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