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p404-Grx5-GFP1-10
(Plasmid #91952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p404TDH3
  • Backbone manufacturer
    PMID: 7737504
  • Vector type
    Yeast Expression
  • Selectable markers
    CloNat/Amp/TRP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grx5-GFP1-10
  • Species
    S. cerevisiae (budding yeast)
  • Promoter GAP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Split GFP was synthesized using gBlocks based on these references: Stephanie Cabantous et al., 2005 Nature Methods and Christine J. Smoyer et al. 2016 JCB.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p404-Grx5-GFP1-10 was a gift from Rong Li (Addgene plasmid # 91952 ; http://n2t.net/addgene:91952 ; RRID:Addgene_91952)

  • For your References section:

    Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R. Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695. Epub 2017 Mar 1. 10.1038/nature21695 PubMed 28241148
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