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Purpose(Empty Backbone) Universal C-terminal GFP11 tagging
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a-GFP(S65T)-His3Mx6
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Backbone manufacturerKurt Thorn
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Vector typeYeast Expression
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Selectable markersHis3 /Amp
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Split GFP was synthesized using gBlocks based on these references: Stephanie Cabantous et al., 2005 Nature Methods and Christine J. Smoyer et al. 2016 JCB.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-GFP11-His3MX was a gift from Rong Li (Addgene plasmid # 91951 ; http://n2t.net/addgene:91951 ; RRID:Addgene_91951) -
For your References section:
Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R. Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695. Epub 2017 Mar 1. 10.1038/nature21695 PubMed 28241148