pAAV-CBh-DIO-CB2-EGFP
(Plasmid #91946)

• Purpose: Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre
• Depositing Lab: Jimok Kim
• Publication: Li et al Neuroscience. 2017 Sep 6. pii: S0306-4522(17)30629-2. doi: 10.1016/j.neuroscience.2017.08.053.

Backbone

• Vector backbone: pAAV-Ef1a-DIO-EGFP-WPRE-pA
• Backbone manufacturer: K. Deisseroth Lab (Addgene #37084)
• Backbone size w/o insert (bp): 6342
• Total vector size (bp): 6484
• Modifications to backbone: 1. The EF1α promoter between the restriction sites MluI and BamHI in the backbone plasmid was replaced by the CBh promoter, which was PCR-amplified from the plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230). 2. The WPRE-pA between EcoRI and BstEII sites in the backbone plasmid was replaced by the CW3SL sequence, which was amplified by PCR from the plasmid pAAV-CW3SL-EGFP (Addgene #61463). 3. The mouse Cnr2 gene was PCR-amplified from the plasmid MR205238L2 (Origene) with primers containing the sequences of FLAG and P2A, and then, inserted into the NheI (BmtI) site downstream of the eGFP sequence in the control plasmid.
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: EGFP
• Species: Synthetic
• Insert Size (bp): 846
• Tag / Fusion Protein:
  • None

Gene/Insert 2

• Gene/Insert name: type-2 cannabinoid receptor
• Alt name: CB2 cannabinoid receptor
• Alt name: CB2R
• Species: M. musculus (mouse)
• Insert Size (bp): 1041
• Tag / Fusion Protein:
  • FLAG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry