pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635
(Plasmid
#91918)
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PurposeExpresses LbfCas13a for bacterial expression and contains backbone for spacer cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 91918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYC184
- Backbone size w/o insert (bp) 4196
- Total vector size (bp) 7479
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLbfCas13a and guide expression cassette
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SpeciesListeriaceae bacterium FSL M6-0635
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Insert Size (bp)3283
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Addgene's quality control sequencing suggests that there may be a mixed population of LbfCas13a Ala727 and Thr727.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635 was a gift from Feng Zhang (Addgene plasmid # 91918 ; http://n2t.net/addgene:91918 ; RRID:Addgene_91918) -
For your References section:
RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959
