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pC019 - LwCas13a from Leptotrichia wadei
(Plasmid #91909)

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Item Catalog # Description Quantity Price (USD)
Plasmid 91909 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pACYC184
  • Backbone size w/o insert (bp) 4197
  • Total vector size (bp) 7783
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LwCas13a and guide expression cassette
  • Alt name
    LwaCas13a
  • Species
    Leptotrichia wadei
  • Insert Size (bp)
    3586

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC019 - LwCas13a from Leptotrichia wadei was a gift from Feng Zhang (Addgene plasmid # 91909 ; http://n2t.net/addgene:91909 ; RRID:Addgene_91909)

  • For your References section:

    RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959
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