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Addgene

pC017 - LwCas13a guide expression backbone with tRNAval promoter Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pC017 - LwCas13a guide expression backbone with tRNAval promoterwith the following enzymes: EcoRI-HF, PvuI-HF, and SacII.

EcoRI-HF: 1 band: 2700 bp
PvuI-HF/SacII: 2 bands: 2462bp and 304bp