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pC012 LwCas13a locus with nontargeting spacer
(Plasmid #91904)

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Item Catalog # Description Quantity Price (USD)
Plasmid 91904 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pACYC184
  • Backbone size w/o insert (bp) 4153
  • Total vector size (bp) 7791
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LwCas13a with non-targeting spacer
  • Alt name
    LwaCas13a
  • Species
    Leptotrichia wadeii
  • Insert Size (bp)
    3456

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC012 LwCas13a locus with nontargeting spacer was a gift from Feng Zhang (Addgene plasmid # 91904 ; http://n2t.net/addgene:91904 ; RRID:Addgene_91904)

  • For your References section:

    Nucleic acid detection with CRISPR-Cas13a/C2c2. Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321. 10.1126/science.aam9321 PubMed 28408723
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