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Addgene

pC014 - LwCas13a-msfGFP Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extend DNA ladder and a digestion of plasmid 91902.

We predict the following pattern:

PvuI-HF: 13.6 kb
NheI-HF: 13.6 kb
PvuI-HF/NheI-HF: 9.2kb & 4.4kb

We see the expected result.