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pC010 LshCas13a locus with nontargeting spacer
(Plasmid #91901)

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Item Catalog # Description Quantity Price (USD)
Plasmid 91901 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pACYC184
  • Backbone size w/o insert (bp) 4153
  • Total vector size (bp) 8486
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LshCas13a locus with non-targeting spacer
  • Species
    leptotrichia shahii
  • Insert Size (bp)
    4333

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene's quality control sequencing identified an R820W amino acid residue substitution in the LshCas13a insert. This residue change is not thought to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC010 LshCas13a locus with nontargeting spacer was a gift from Feng Zhang (Addgene plasmid # 91901 ; http://n2t.net/addgene:91901 ; RRID:Addgene_91901)

  • For your References section:

    Nucleic acid detection with CRISPR-Cas13a/C2c2. Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321. 10.1126/science.aam9321 PubMed 28408723
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