MIG-REST
(Plasmid #91898)

• Purpose: Expression of mouse REST
• Depositing Lab: Julien Sage
• Publication: MIG-REST (unpublished)

Backbone

• Vector backbone: MIGR1
• Backbone manufacturer: Warren Pear
• Backbone size w/o insert (bp): 6231
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: REST
• Alt name: NRSF
• Alt name: RE1-Silencing Transcription Factor
• Alt name: Neuron-Restrictive Silencer Factor
• Species: M. musculus (mouse)
• Insert Size (bp): 3660
• GenBank ID: NM_011263
• Entrez Gene: Rest (a.k.a. 2610008J04Rik, NRSF, REST4)
• Promoter: MSCV
• Tag / Fusion Protein:
  • ires-GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert DNA for NRSF obtained from Addgene 21310 pHR'-NRSF-CITE-GFP by BamHI and EcoRI digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry