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Addgene

pLIX-hN1ICD
(Plasmid #91897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIX_403
  • Backbone manufacturer
    David Root lab
  • Backbone size w/o insert (bp) 9396
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Notch1 intracellular domain
  • Alt name
    N1ICD
  • Alt name
    Notch1-ICD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2360
  • Mutation
    codons 1770 to 2555 of human NOTCH1
  • GenBank ID
  • Entrez Gene
    NOTCH1 (a.k.a. AOS5, AOVD1, TAN1, hN1)
  • Promoter TRE promoter, Tet ON

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer O.PGK1b-R (GAACGGACGTGAAGAATGTG)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert DNA obtained by PCR from MIG-NICD plasmid (Dr. Warren Pear)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIX-hN1ICD was a gift from Julien Sage (Addgene plasmid # 91897 ; http://n2t.net/addgene:91897 ; RRID:Addgene_91897)
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