MSCV-mouse Ezh2 Y637S-IRES-GFP
(Plasmid #91887)

• Purpose: Retroviral expression of mouse Ezh2 with Y637S
• Depositing Lab: Martine Roussel
• Publication: Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917. doi: 10.1016/j.celrep.2017.02.073.

Backbone

• Vector backbone: MSCV-IRES-GFP
• Backbone manufacturer: Tannishtha Reya (Addgene #20672)
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ezh2
• Species: M. musculus (mouse)
• Mutation: Y637S
• Entrez Gene: Ezh2 (a.k.a. Enx-1, Enx1h, KMT6, mKIAA4065)
• Promoter: MMLV gag
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pLXSN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's QC finds the Y->S substitution at residue 641 (rather than 637). This is the correct location for this isoform and the plasmid should function as described in the publication.