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p2CT-His-MBP-Lbu_C2c2_E299A
(Plasmid #91855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2CT
  • Backbone manufacturer
    UC Berkeley Macrolab
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LbuCas13a (LbuC2c2) E299A
  • Mutation
    E299A
  • Tag / Fusion Protein
    • His6-MBP-TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2CT-His-MBP-Lbu_C2c2_E299A was a gift from Jennifer Doudna (Addgene plasmid # 91855 ; http://n2t.net/addgene:91855 ; RRID:Addgene_91855)

  • For your References section:

    RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008. 10.1016/j.molcel.2017.04.008 PubMed 28475872
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