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pGL4.23-CD69 CaRE3 (minus strand)
(Plasmid #91845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.23
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4283
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD69 CaRE3 (minus strand)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    560
  • Mutation
    Missing 1 base from 13 base polyT tract beginning at chr12:9926298
  • Entrez Gene
    CD69 (a.k.a. AIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned from Jurkat gDNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.23-CD69 CaRE3 (minus strand) was a gift from Jacob Corn (Addgene plasmid # 91845 ; http://n2t.net/addgene:91845 ; RRID:Addgene_91845)

  • For your References section:

    Discovery of stimulation-responsive immune enhancers with CRISPR activation. Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, Marson A. Nature. 2017 Sep 7;549(7670):111-115. doi: 10.1038/nature23875. Epub 2017 Aug 30. 10.1038/nature23875 PubMed 28854172
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