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Addgene

pDD401
(Plasmid #91828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pmyo-2::GFP
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2233
  • Mutation
    Nucleotide 489 of the myo-2 promoter was changed from G to C to eliminate a SapI site

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Pmyo-2::GFP cassette is inserted in place of a fluorescent protein in the assembled repair template. This is intended for knocking out a gene and replacing it with Pmyo-2::GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD401 was a gift from Bob Goldstein (Addgene plasmid # 91828 ; http://n2t.net/addgene:91828 ; RRID:Addgene_91828)

  • For your References section:

    SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette. Dickinson D, Slabodnick M, Chen A, Goldstein B. MicroPubl Biol. 2018 May 1;2018:10.17912/W2KT0N. doi: 10.17912/W2KT0N. 10.17912/W2KT0N PubMed 32550377
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