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CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)
(Plasmid #91820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 7771
  • Total vector size (bp) 13039
  • Modifications to backbone
    Addition of endogenous RPB1 promoter and 3’ untranslated regions
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RPO21
  • Alt name
    RPB1, RPB220, SUA8, B220
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    5268
  • Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP), and 10xHis tag inserted between residues P1455 and E1456; changed lysine 1487 to asparagine and aspartate 1497 to lysine
  • GenBank ID
    NM_001180200.1
  • Entrez Gene
    RPO21 (a.k.a. YDL140C, RPB1, RPB220, SIT1, SUA8)
  • Promoter Endogenous Rpb1 promoter
  • Tag / Fusion Protein
    • 10xHis

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sph1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer CTGTTTCCAACACCCAT
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2) was a gift from Chris Hill (Addgene plasmid # 91820 ; http://n2t.net/addgene:91820 ; RRID:Addgene_91820)

  • For your References section:

    A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription. Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, Whitby FG, Formosa T, Hill CP. Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723. 10.7554/eLife.28723 PubMed 28826505
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