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CPH1141 (pET151_Spt6 1247-1451)
(Plasmid #91815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET151/D-TOPO
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5766
  • Total vector size (bp) 6381
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPT6
  • Alt name
    CRE2
  • Alt name
    SSN20
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    615
  • Mutation
    deleted residues 1-1246
  • GenBank ID
    NM_001181245.1
  • Entrez Gene
    SPT6 (a.k.a. YGR116W, CRE2, SSN20)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CPH1141 (pET151_Spt6 1247-1451) was a gift from Chris Hill (Addgene plasmid # 91815 ; http://n2t.net/addgene:91815 ; RRID:Addgene_91815)

  • For your References section:

    A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription. Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, Whitby FG, Formosa T, Hill CP. Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723. 10.7554/eLife.28723 PubMed 28826505
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