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PurposeConstitutive expression of creGFP fusion using PGK promoter in a lentiviral backbone.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRL
- Backbone size w/o insert (bp) 8483
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre-GFP fusion protein
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Alt nameCre recombinase
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene Plasmid #12252 replacing GFP with the creGFP fusion sequence from Addgene Plasmid #13776.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRlsinPGK_CREGFP_WPRE was a gift from Beverly Torok-Storb (Addgene plasmid # 91789 ; http://n2t.net/addgene:91789 ; RRID:Addgene_91789)