pRRlsinPGK_H2BGFP_WPRE
(Plasmid #91788)

• Purpose: Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.
• Depositing Lab: Beverly Torok-Storb
• Publication: Torok-Storb lab lentiviral fluorescent protein (unpublished)

Backbone

• Vector backbone: pRRL
• Backbone manufacturer: Trono lab
• Backbone size w/o insert (bp): 7840
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human histone H2B
• Species: H. sapiens (human)
• Entrez Gene: H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Plasmid #12252 replacing GFP with the H2B-GFP fusion sequence from Addgene Plasmid #11680.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry