pRRlsinPGK_dectintagBFP2_WPRE
(Plasmid #91786)

• Purpose: Constitutive expression of mTagBFP2-Dectin1A-N-10 using PGK promoter in a lentiviral backbone.
• Depositing Lab: Beverly Torok-Storb
• Publication: Torok-Storb lab lentiviral fluorescent protein (unpublished)

Backbone

• Vector backbone: pRRL
• Backbone manufacturer: Trono lab
• Backbone size w/o insert (bp): 8179
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dectin1A
• Alt name: NP_922938.1
• Species: H. sapiens (human)
• Entrez Gene: CLEC7A (a.k.a. BGR, CANDF4, CD369, CLECSF12, DECTIN1, SCARE2)
• Tag / Fusion Protein:
  • mTagBFP2 (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Plasmid #12252 replacing GFP with the mTagBFP2-Dectin1A-N-10 sequence from Addgene Plasmid #55291.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry