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Addgene

pBAD IFP1.4
(Plasmid #91775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pBAD (+ HO-1)
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    0.02% (weight/volume) of Arabinose is necessary for fluorescent protein production.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Infrared Fluorescent Protein 1.4 + Heme oxygenase-1
  • Alt name
    IFP1.4
  • Alt name
    HO-1
  • Species
    Deinococcus radiodurans (Bacterial phytocrhome, IFP1.4) + Synechocytis (Cyanobacteria, HO-1)
  • Insert Size (bp)
    1755
  • Promoter araBAD
  • Tag / Fusion Protein
    • Hexa-Histidine tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBAD Forward
  • 3′ sequencing primer unecessary
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD IFP1.4 was a gift from Erik Rodriguez & Roger Tsien (Addgene plasmid # 91775 ; http://n2t.net/addgene:91775 ; RRID:Addgene_91775)

  • For your References section:

    Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY. Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683. 10.1126/science.1168683 PubMed 19423828
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