pBAD mPlum
(Plasmid #91773)

• Purpose: Bacterial expression of a fluorescent protein, monomeric Plum (mPlum)
• Depositing Labs: Erik Rodriguez, Roger Tsien
• Publication: Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov 19.

Backbone

• Vector backbone: pBAD
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 4000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 0.02% (weight/volume) of Arabinose is necessary for fluorescent protein production.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: monomeric Plum
• Alt name: mPlum
• Species: Discosoma sp. (mushroom coral)
• Insert Size (bp): 735
• Promoter: araBAD
• Tag / Fusion Protein:
  • no tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBAD Forward
• 3′ sequencing primer: unecessary

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.