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pFUSEss-CHIg-mM-Asp212Ala_M18
(Plasmid #91735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUSEss-CHIg-mM
  • Backbone manufacturer
    Invivogen
  • Backbone size w/o insert (bp) 4852
  • Total vector size (bp) 5219
  • Modifications to backbone
    Nucleotides coding for Asp212 (EU numbering) were changed into triplet encoding Ala.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Top10 or DH5alpha could be selected on LB LOW SALT with 35-50 micrograms/ml Zeocin.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    immunoglobulin heavy constant mu
  • Alt name
    IgM, IGHM, mouse IgM (immunoglobulin M) heavy chain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    367
  • Mutation
    Asp212 changed to Ala (EU numbering)
  • Entrez Gene
    Ighm (a.k.a. Igh-6, Igh-M, Igh6, Igm, muH)
  • Promoter hEF1-HTLV prom

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUSEss-CHIg-mM-Asp212Ala_M18 was a gift from Joanna Bereta (Addgene plasmid # 91735 ; http://n2t.net/addgene:91735 ; RRID:Addgene_91735)

  • For your References section:

    Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Klaus T, Stalinska K, Czaplicki D, Mak P, Skupien-Rabian B, Kedracka-Krok S, Wiatrowska K, Bzowska M, Machula M, Bereta J. Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1038/s41598-017-19003-4. 10.1038/s41598-017-19003-4 PubMed 29323348
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