pTTP1B
(Plasmid
#91723)
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Purpose(Empty Backbone) integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 91723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMOSBlue
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Vector typeintegration-proficient plasmid vectors for mycobacteria
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmids pTTP1A and pTTP1B differ in regard to the orientation of the attP–int region with respect to the plasmid backbone.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTTP1B was a gift from Graham Hatfull (Addgene plasmid # 91723 ; http://n2t.net/addgene:91723 ; RRID:Addgene_91723) -
For your References section:
Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria. Pham TT, Jacobs-Sera D, Pedulla ML, Hendrix RW, Hatfull GF. Microbiology. 2007 Aug;153(Pt 8):2711-23. 10.1099/mic.0.2007/008904-0 PubMed 17660435
