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Purpose(Empty Backbone) Integration-proficient vector for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 91720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC119
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the attP-int region from Mycobacteriophage L5, a temperate phage of mycobacteria.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH94 was a gift from Graham Hatfull (Addgene plasmid # 91720 ; http://n2t.net/addgene:91720 ; RRID:Addgene_91720) -
For your References section:
Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin. Lee MH, Pascopella L, Jacobs WR Jr, Hatfull GF. Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3111-5. PubMed 1901654
