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CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV
(Plasmid #91719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91719 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone size w/o insert (bp) 4650
  • Total vector size (bp) 5775
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mEGFP-P2A-paAIP2(R5AR6A)
  • Species
    Synthetic
  • Insert Size (bp)
    1239
  • Promoter CaMKII 1.3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AseI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer gaccccaacgagaagcgcg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV was a gift from Ryohei Yasuda (Addgene plasmid # 91719 ; http://n2t.net/addgene:91719 ; RRID:Addgene_91719)

  • For your References section:

    Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Murakoshi H, Shin ME, Parra-Bueno P, Szatmari EM, Shibata AC, Yasuda R. Neuron. 2017 Apr 5;94(1):37-47.e5. doi: 10.1016/j.neuron.2017.02.036. Epub 2017 Mar 16. 10.1016/j.neuron.2017.02.036 PubMed 28318784
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