CaMKIIP-mEGFP-P2A-paAIP2/pAAV
(Plasmid #91718)

• Purpose: AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII
• Depositing Lab: Ryohei Yasuda
• Publication: Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi: 10.1016/j.neuron.2017.02.036. Epub 2017 Mar 16.

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Backbone

• Vector backbone: pAAV-MCS
• Backbone size w/o insert (bp): 4650
• Total vector size (bp): 5775
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mEGFP-P2A-paAIP2
• Species: Synthetic
• Insert Size (bp): 1239
• Promoter: CaMKII 1.3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AseI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: gaccccaacgagaagcgcg

Resource Information

• Supplemental Documents:
  • GenBank CaMKIIP-mEGFP-P2A-paAIP2/pAAV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry