pBSE901
(Plasmid
#91709)
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Purpose(Empty Backbone) CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 91709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCambia
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSE901 was a gift from Qi-Jun Chen (Addgene plasmid # 91709 ; http://n2t.net/addgene:91709 ; RRID:Addgene_91709) -
For your References section:
CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. 10.1007/s11427-017-9021-5 [pii] PubMed 28303459
