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Purpose(Empty Backbone) CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCambia
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHEE901 was a gift from Qi-Jun Chen (Addgene plasmid # 91707 ; http://n2t.net/addgene:91707 ; RRID:Addgene_91707) -
For your References section:
CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. 10.1007/s11427-017-9021-5 [pii] PubMed 28303459