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7TFP CDH1 mutant reporter
(Plasmid #91703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    7TFP
  • Backbone manufacturer
    Roel Nusse (Addgene plasmid # 24308)
  • Backbone size w/o insert (bp) 9061
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDH1 mutant promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    243
  • Mutation
    mutant E-boxes
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
  • Promoter CDH1 mutant
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sbf1 (unknown if destroyed)
  • 3′ cloning site Bmt1 (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7TFP CDH1 mutant reporter was a gift from Bob Weinberg (Addgene plasmid # 91703 ; http://n2t.net/addgene:91703 ; RRID:Addgene_91703)

  • For your References section:

    Activation of PKA leads to mesenchymal-to-epithelial transition and loss of tumor-initiating ability. Pattabiraman DR, Bierie B, Kober KI, Thiru P, Krall JA, Zill C, Reinhardt F, Tam WL, Weinberg RA. Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680. 10.1126/science.aad3680 PubMed 26941323
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