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LZ10 PBREBAC-H2BHalo
(Plasmid #91564)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    synthetic
  • Total vector size (bp) 6652
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    histone H2B-HaloTag
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1293
  • Promoter EF1
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer gene-specific primers
  • 3′ sequencing primer gene-specific primers
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZ10 PBREBAC-H2BHalo was a gift from James Zhe Liu (Addgene plasmid # 91564 ; http://n2t.net/addgene:91564 ; RRID:Addgene_91564)

  • For your References section:

    Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. Li L, Liu H, Dong P, Li D, Legant WR, Grimm JB, Lavis LD, Betzig E, Tjian R, Liu Z. Elife. 2016 Aug 3;5. pii: e17056. doi: 10.7554/eLife.17056. 10.7554/eLife.17056 PubMed 27484239
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