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Addgene

pHH0103_EPS8L2-1/1
(Plasmid #91526)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91526 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHH0103
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EPS8L2-1/1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    221
  • Entrez Gene
    EPS8L2 (a.k.a. EPS8R2, MGC3088, FLJ16738, FLJ21935, FLJ22171, MGC126530)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sfi1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found R534C within the EPS8L2 translation compared to NP_073609.2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHH0103_EPS8L2-1/1 was a gift from Sachdev Sidhu (Addgene plasmid # 91526 ; http://n2t.net/addgene:91526 ; RRID:Addgene_91526)

  • For your References section:

    Comprehensive Analysis of the Human SH3 Domain Family Reveals a Wide Variety of Non-canonical Specificities. Teyra J, Huang H, Jain S, Guan X, Dong A, Liu Y, Tempel W, Min J, Tong Y, Kim PM, Bader GD, Sidhu SS. Structure. 2017 Oct 3;25(10):1598-1610.e3. doi: 10.1016/j.str.2017.07.017. Epub 2017 Sep 7. 10.1016/j.str.2017.07.017 PubMed 28890361
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