pl-Synapsin-YFP-NaVII-III
(Plasmid #91246)

• Purpose: Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)
• Depositing Lab: Matthew Grubb
• Publication: Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268. eCollection 2016.

Backbone

• Vector backbone: pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
• Backbone manufacturer: Addgene plasmid 20945
• Backbone size w/o insert (bp): 10093
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nav1.2
• Alt name: sodium channel subunit Nav1.2
• Alt name: sodium channel, voltage-gated
• Species: R. norvegicus (rat)
• Insert Size (bp): 684
• Mutation: The insert codes only for the intracellular loop between transmembrane domains II and III
• Entrez Gene: Scn2a (a.k.a. NachII, Nav1.2, RII/RIIA, RNSCPIIR, SCN, Scn2a1, Scn2a2, ScpII)
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The backbone was constructed by Karl Deisseroth at Stanford and is available at Addgene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the linked article - Dumitrescu et al. (2016) - for full details of the cloning procedure used to generate this consruct