pACYCDuet_W22HDHFR_WTTS
(Plasmid
#91241)
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Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYCDuet-1
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namefolA
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Alt nameDHFR
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SpeciesE. coli
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MutationW22H
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namethyA
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SpeciesE.coli
- Promoter T7
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACYCDuet_W22HDHFR_WTTS was a gift from Kimberly Reynolds (Addgene plasmid # 91241 ; http://n2t.net/addgene:91241 ; RRID:Addgene_91241) -
For your References section:
A Two-Enzyme Adaptive Unit within Bacterial Folate Metabolism. Schober AF, Mathis AD, Ingle C, Park JO, Chen L, Rabinowitz JD, Junier I, Rivoire O, Reynolds KA. Cell Rep. 2019 Jun 11;27(11):3359-3370.e7. doi: 10.1016/j.celrep.2019.05.030. 10.1016/j.celrep.2019.05.030 PubMed 31189117