pACYCDuet_G121VDHFR_WTTS
(Plasmid #91233)

• Purpose: bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
• Depositing Lab: Kimberly Reynolds
• Publication: Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e7. doi: 10.1016/j.celrep.2019.05.030.

Backbone

• Vector backbone: pACYCDuet-1
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: folA
• Alt name: DHFR
• Species: E. coli
• Mutation: G121V
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: thyA
• Alt name: TS
• Species: E.coli
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pACYCDuet_G121VDHFR_WTTS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry