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pACYCDuet_F31YG121VDHFR_R166QTS
(Plasmid #91228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 91228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYCDuet-1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    folA
  • Alt name
    DHFR
  • Species
    E. coli
  • Mutation
    F31YG121
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    thyA
  • Alt name
    TS
  • Species
    E.coli
  • Mutation
    R166Q
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYCDuet_F31YG121VDHFR_R166QTS was a gift from Kimberly Reynolds (Addgene plasmid # 91228 ; http://n2t.net/addgene:91228 ; RRID:Addgene_91228)

  • For your References section:

    A Two-Enzyme Adaptive Unit within Bacterial Folate Metabolism. Schober AF, Mathis AD, Ingle C, Park JO, Chen L, Rabinowitz JD, Junier I, Rivoire O, Reynolds KA. Cell Rep. 2019 Jun 11;27(11):3359-3370.e7. doi: 10.1016/j.celrep.2019.05.030. 10.1016/j.celrep.2019.05.030 PubMed 31189117
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