pMOD_B2203
(Plasmid #91066)

• Purpose: Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C
• Depositing Lab: Daniel Voytas
• Publication: Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.

Backbone

• Vector backbone: pMOD_B2000
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
• Species: Synthetic
• Promoter: CmYLCV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers - combinable with another array of multiple gRNA spacers in MODULE C, Terminator: none (will be fused to gRNA array in MODULE C)