mApple-Sec61b-C1
(Plasmid #90993)

• Purpose: Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells
• Depositing Lab: Jennifer Lippincott-Schwartz
• Publication: Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf3928. Epub 2016 Oct 27.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4732
• Total vector size (bp): 5000
• Modifications to backbone: Cloned from pAc-GFPC1-Sec61beta (Addgene # 15108) through AgeI and BsrG1 sites to change the color of the fluorescent protein.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mApple-Sec61b
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1014
• Entrez Gene: SEC61B
• Promoter: CMV
• Tag / Fusion Protein:
  • mApple (red fluorescence) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BsrG1 (not destroyed)
• 5′ sequencing primer: CMVfor
• 3′ sequencing primer: SV40polyA

Resource Information

• Supplemental Documents:
  • mApple-Sec61b-C1.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: The plasmid is modified from pAcGFP-C1-Sec61b, generated by Tom Rapoport's Lab (Harvard). It was acquired by Craig Blackstone's Lab (NIH\NINDS), and modified in Jennifer Lippincott-Schwartz's Lab (NIH\NICHD).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry