pADH137
(Plasmid
#90986)
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PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90986 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2242
- Total vector size (bp) 9184
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Vector typeCRISPR
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Selectable markersNourseothricin (NAT)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameC. albicans LEU2 1 of 2
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Insert Size (bp)750
Gene/Insert 2
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Gene/Insert namepENO1
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Insert Size (bp)783
Gene/Insert 3
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Gene/Insert nameCas9
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Insert Size (bp)4113
Gene/Insert 4
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Gene/Insert nameNAT 1 of 2
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Insert Size (bp)842
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pADH137 was a gift from Aaron Hernday (Addgene plasmid # 90986 ; http://n2t.net/addgene:90986 ; RRID:Addgene_90986) -
For your References section:
An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans. Nguyen N, Quail MMF, Hernday AD. mSphere. 2017 Apr 26;2(2):e00149-17. doi: 10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr. 10.1128/mSphereDirect.00149-17 PubMed 28497115
Map uploaded by the depositor.
