pADH99
(Plasmid #90979)

• Purpose: NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
• Depositing Lab: Aaron Hernday
• Publication: Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi: 10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2242
• Total vector size (bp): 11119
• Vector type: CRISPR
• Selectable markers: Nourseothricin (NAT)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: US_HIS1
• Insert Size (bp): 433

Gene/Insert 2

• Gene/Insert name: FRT
• Insert Size (bp): 34

Gene/Insert 3

• Gene/Insert name: pENO1
• Insert Size (bp): 783

Gene/Insert 4

• Gene/Insert name: C. albicans Cas9
• Insert Size (bp): 4113

Gene/Insert 5

• Gene/Insert name: pMAL2
• Insert Size (bp): 548

Gene/Insert 6

• Gene/Insert name: FLP
• Insert Size (bp): 1272

Gene/Insert 7

• Gene/Insert name: NAT 1 of 2
• Insert Size (bp): 846

Resource Information

• Supplemental Documents:
  • padh99.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry