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Addgene

pRS313-PRP8
(Plasmid #90964)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS313
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRP8
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)
  • Promoter native

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site SnaBI (unknown if destroyed)
  • 5′ sequencing primer gene specific
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kuhn and Brow, Genetics 155: 1667–1682; Price et al., RNA. 2014 Jan;20(1):46-60

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS313-PRP8 was a gift from Christine Guthrie (Addgene plasmid # 90964 ; http://n2t.net/addgene:90964 ; RRID:Addgene_90964)

  • For your References section:

    Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency. Mayerle M, Raghavan M, Ledoux S, Price A, Stepankiw N, Hadjivassiliou H, Moehle EA, Mendoza SD, Pleiss JA, Guthrie C, Abelson J. Proc Natl Acad Sci U S A. 2017 Apr 17. pii: 201701462. doi: 10.1073/pnas.1701462114. 10.1073/pnas.1701462114 PubMed 28416677
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