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Addgene

pSG5 Large T K1
(Plasmid #9055)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 9055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    large T antigen K1
  • Alt name
    large T antigen
  • Alt name
    large T cDNA
  • Species
    simian virus
  • Insert Size (bp)
    2200
  • Mutation
    E107K. Mutation in pRb LxCxE binding domain.
  • Entrez Gene
    SV40gp6 (a.k.a. SV40gp6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference: Zalvide et al. Mol Cell Biol 15(10). p. 5800-5810.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5 Large T K1 was a gift from William Hahn (Addgene plasmid # 9055 ; http://n2t.net/addgene:9055 ; RRID:Addgene_9055)
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