pBABE puro K-Ras V12
(Plasmid #9052)

• Purpose: Retroviral expression of a constitutively active form of K-Ras
• Depositing Lab: William Hahn
• Publication: Viral vectors. (unpublished)

Backbone

• Vector backbone: pBabe puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KRAS
• Species: H. sapiens (human)
• Insert Size (bp): 570
• Mutation: G12V. Constitutively active.
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 25 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry