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PurposeCas9 gap repair vector - HIS3
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90519 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbone2 micron
- Backbone size w/o insert (bp) 3882
- Total vector size (bp) 11132
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Vector typeYeast Expression, CRISPR
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
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SpeciesS. cerevisiae (budding yeast)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information see https://benchling.com/pub/ellis-crispr-tools
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWS172 was a gift from Tom Ellis (Addgene plasmid # 90519 ; http://n2t.net/addgene:90519 ; RRID:Addgene_90519)
Map uploaded by the depositor.
